- Catalog numberMBS673129
- Product nameHamster Anti-Mouse CD3e-FITC
- Size5x0.5 mg
- PriceAsk For Price
- Products typeAntibody
- Products short name[CD3 epsilon]
- Products name syn[Hamster Anti-Mouse CD3e - FITC; Hamster Anti-Mouse CD3 epsilon]
- Other names[CD3 epsilon; CD3 epsilon]
- Other gene names[cd3e]
- ClonalityMonoclonal
- IsotypeArmenian Hamster IgG kappa
- Clone[145-2C11]
- HostArmenian Hamster
- ReactivityMouse
- SpecificityMouse CD3 epsilon (Mr 25 kDa) CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145- 2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death. ^1-6
- FormFluorescein (FITC) Conjugate
- Storage stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Alexa Fluor 647 (AF647) conjugate is supplies as 0.1 mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
- Tested applicationFlow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot
- Alternative to gene target
- [ "CD3e molecule, epsilon (CD3-TCR complex)", "IMD18 and T3E and TCRE", "CD3E and IDBG-73617 and ENSG00000198851 and 916", "protein heterodimerization activity", "Cell surfaces", "Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501", "CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054" ]
- Gene targetCD3e
- Identity:HGNC:1674
- Gene:CD3E
- Long gene name:CD3e molecule
- Synonyms gene name:CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex)
- Discovery year:1986-01-01
- Entrez gene record:916
- RefSeq identity:NM_000733
- Classification:CD molecules
- VEGA ID:OTTHUMG00000166968
- Identity:HGNC:24219
- Gene:POLR1G
- Long gene name:RNA polymerase I subunit G
- Synonyms gene name:CD3e antigen, epsilon polypeptide associated protein, CD3e molecule associated protein
- Synonyms:ASE-1, CASTPAF49RPA34A34.5
- Discovery year:2005-04-21
- Entrez gene record:10849
- Pubmed identification:10373416, 9426281, 15226435, 23150253
- RefSeq identity:NM_012099
- Classification:RNA polymerase subunits
- VEGA ID:OTTHUMG00000182120
- Gene symbolCD3E, POLR1G
- isotype filter
- Armenian Hamster IgG kappa
- Short nameAnti-Mouse CD3e-FITC
- LabelFITC
- label filter
- FITC
- technique filter
- anti-
- Mouse
- anti
- FITC
- Techniqueanti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
- Alternative techniquemurine, antibodies, fluorescine
Gene info
Locus Specific Databases:CD3Ebase: Mutation registry for autosomal recessive CD3epsilon immunodeficiencyLRG_38