- Catalog numberMBS571291
- Product nameGoat anti Chicken IgM (Fc specific), conjugated with FITC
- Size1 mililiter
- PriceAsk For Price
- Alternative name1Anti-Goat anti Chicken IgM (Fc specific), conjugated with FITC
- Alternative name2Caprine anti Chicken IgM (Fc specific), conjugated with FITC
- Alternative name3Caprinae anti Gallus gallus IgM (Fc specific), conjugated with FITC
- Alternative name4Goat anti Chicken IgM (Fc specific)
- Alternative name5N/A
- Other namesN/A
- Gene nameN/A
- Gene name synonimsN/A
- Other gene namesN/A
- CategorySecondary Antibodies
- ClonalityPolyclonal
- Immunoglobulin isotypeN/A
- ClonePolyclonal antibody
- Host organismGoat
- Species reactivityThis immunoconJugate is not species-specific since Inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiserum has not been tested in detail.
- Specificity and cross reactivityThe reactivity of the antiserum IS directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against chicken serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as Immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in chicken serum. as tested by Immunoelectrophoresis and double radial immunodiffusion.
- Purification methodHyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It IS free of other serum proteins as tested by immunoelectrophoresis
- Form AppearanceFITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- ConcentrationN/A
- Storage and shippingHandling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4'C; prolonged storage at or below -20'C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20'C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS. pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4'C. not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Packing: Vial with 1 mllyophilized immunoconjugate. Storage and Shelf life: Lyophilized at +4'C = at least 10 years reconstituted at or below -20'C =3-5 years reconstituted at +4 'C =7 days_x000D_ _x000D_ _x000D_
- Tested forELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
- Descriptionproductone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
- AdvisoryIn order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
- Gene targetFc specific conjugated with
- HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
- IsotypeIgM, IgM
- isotype filter
- IgM
- Short nameGoat anti IgM (Fc specific), conjugated with FITC
- LabelFITC
- label filter
- FITC
- technique filter
- anti
- FITC
- Goat
- IgM
- FC
- Techniqueanti, FITC, Goat, IgM, FC, antibody to, antibody Conjugates, Fluorescein, goats, IgMs
- Alternative techniqueantibodies, caprine, fluorescine, immunoglobulin-m