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IVD Over-expression Lysate reagent

  • Catalog number
    bs-2752R-Cy5.5
  • Product name
    BTK Antibody, Cy5.5 Conjugated
  • Size
    0.1ml
  • Short name
    Anti-BTK
  • Long name
    BTK Polyclonal Antibody, Cy5.5 Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Cy5.5
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    BTK
  • Specificity
    This is a highly specific antibody against BTK.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic peptide derived from human BTK
  • Gene ID number
    695
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/BTK inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal BTK activity.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    675nm/694nm
  • Synonyms
    Agammaglobulinaemia tyrosine kinase; AGMX 1; AGMX1; AT; ATK; B cell progenitor kinase; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton’s Tyrosine Kinase; Btk; BTK_HUMAN; IMD 1; IMD1; MGC126261; MGC126262; OTTHUMP00000063593; PSCTK 1; PSCTK1; Tyrosine protein kinase BTK; Tyrosine-protein kinase BTK; XLA.
  • Alternative to gene target
    • [ "Bruton agammaglobulinemia tyrosine kinase", "AGMX1 and AT and ATK and BPK and IMD1 and PSCTK1 and XLA", "BTK and IDBG-79651 and ENSG00000010671 and 695", "phosphatidylinositol-3", "nuclei", "Btk and IDBG-171427 and ENSMUSG00000031264 and 12229", "BTK and IDBG-633447 and ENSBTAG00000019250 and 533459" ]
  • Gene target
    BTK Conjugated
    • Gene info
    Locus Specific Databases:BTK base: Mutation registry for X-linked agammaglobulinemiaGlobal Variome shared LOVDLRG_128
  • Gene symbol
    BTK
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    Cy5.5
  • label filter
    • Cy5.5
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
  • Alternative technique
    antibodies