• Catalog number
    7904-25
  • Product name
    Bovine Serum Albumin – Cohn Fraction V, pH – 5.2
  • Size
    25 g
  • Description
    Pure Cohn Fraction V BSA, for multiple applications including diagnostic reagents preparation. BSA is prepared by utilizing the Cohn Cold Ethanol Precipitation Process. This process is optimal for preserving the nutrient factors critical to the applications of cell culture, leptospira and microbiological growth. BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids in stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.
  • Product Highlights
    SOURCE: The raw material used to manufacture this lot was derived exclusively from USDA-regulated abattoirs located in the United States. The animals received ante-mortem and post-mortem inspection under a licensed veterinarian’s supervision and were certified at the time of slaughter as free of infectious or contagious diseases and injurious parasites. PURITY: ≥ 98% by SDS-PAGE gel MOL. WEIGHT: ~ 66.43 kDa ENDOTOXIN LEVEL: > 0.3 EU/mg by Gel Clot FORM: Light Brown powder OTHER PARAMETERS: pH (1% solution): 5.2 by pH meter analysis Calcium Content: < 2 mg/dL by Aesenazo III analysis Chloride Content: < 25 mEq/L by ISE analysis Potassium Content: < 0.2 mEq/L by ISE analysis Sodium Content: < 20 mEq/L by ISE analysis Moisture Content: 1.2% by thermogravimetric analysis Microbial Content: Passed lower limit. Growth monitored on a plate at 30-35ºC for 48 hrs. Mycoplasma content: Negative by E.I.A. Qualitative Viral Agents (9 CFR 113): None Detected. SOLUBILITY: 50 mg/ml in water, with 15 min of manual stirring. APPLICATIONS/ADVANTAGES: • Can be used in most systems which do not require a protease free or enzyme free reagent. • Ideal for making diluents, controls and calibrators/standards. • Excellent solubility and ease of filtration
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Datasheet
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Gene target
    Albumin – Cohn Fraction V pH – 5 2
  • Short name
    Serum Albumin – Cohn Fraction V, pH – 5 2
  • technique filter
    • serum
  • Technique
    serum
  • Alternative technique
    sera
  • Tissue
    serum
  • tissue filter
    • serum