anti-Chicken IgY Fc (FITC), donkey polyclonal secondairy antibody

• Catalog number
  DAIgYFc-F
• Price
  Please ask
• Size
  500 ug

• Antigen
  Chicken IgY Fc (FITC)
• Tech file
• Chicken IgY
  Gallus gallus domesticus and other IgY antibodies have high affinity and are easy to collect from the yolk. Gallus gallus can produce 20 more than in rabbit polyclonal. The antibody will not bind to rheumatoid factor or protein or Fc regions. This gives very much reduced background very much suited for diagnostic applications. Papain will digest IgY’s to divalent Fab antibody fragments. IgY’s are suited for Elisa kit development, Immunohistochemistry and Western Blot.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by gallus IgY they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This gallus IgY Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. gallus IgY FITC is highly sensitive to pH extremes. Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
• Latin name
  Equus africanus asinus
• Conjugation
  Anti-FITC Antibody
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  secondairy  
• Short name
  anti- IgY Fc (FITC), polyclonal secondairy antibody
• Technique
  Polyclonal, Antibody, anti-, anti, FITC, IgY, FC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, IgYs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Secondairy antibodies
• Host
  Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Isotype
  IgY antibody
• Label
  FITC
• Species
  chicken, Chickens, Donkeys
• Alternative name
  Antibody toChicken IgY fragment c (fluorecein), donkey polyclonal detector (antibody to-)
• Alternative technique
  polyclonals, antibodies, fluorescine, bird

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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