Bovine IgG FITC
-
Catalog number65C-CH0102
-
PricePlease ask
-
Size1 mg
-
-
CategoryImmunoglobulins
-
Antibody SubtypeConjugated Immunoglobulins
-
Area of researchImmunology
-
ReactivityBovine
-
Conjugate TagFITC
-
Method of PurificationBovine IgG (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
-
Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
-
StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditionsBlue Ice
-
Tested forIF
-
Usage RecommendationsOptimal conditions to be determined by end user
-
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Short nameIgG FITC
-
TechniqueFITC, IgG, Fluorescein, IgGs
-
IsotypeIgG, IgG
-
LabelFITC
-
SpeciesBovine, Bos Taurus genes are available form the Bovine Genome database.
-
Alternative namePurified Bovine IgG FITC conjugate
-
Alternative techniquefluorescine, immunoglobulins
-
MeSH Data
-
Name
-
ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data