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Vimentin monoclonal antibody

  • Catalog number
    11-254-C025
  • Product name
    Vimentin monoclonal antibody
  • Size
    25ug
  • Short name
    Anti-Vimentin Mab
  • Long name
    Mouse Monoclonal Antibody against Mammalian Vimentin, Clone: VI-01
  • Antigen
    Vimentin
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Mammalian
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    VI-01
  • Isotype
    IgM
  • Applications
    WB, ICC
  • Immunogen
    Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
  • Format conjugation
    purified; unconjugated
  • Specificity
    The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin._x000D_
  • Research area
    Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human), Cytoskeleton: Intermediate Filaments
  • Concentration
    1 mg/ml
  • Storage buffer
    Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
  • Shipping and storage
    Mouse Monoclonal Antibody against Mammalian Vimentin, Clone: VI-01 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP)._x000D_ Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin._x000D_ Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by precipitation and chromatography
  • Datasheet
    Datasheet
  • References
    *Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79._x000D_ _x000D_ _x000D_ , *Bacáková L, Mares V, Lisá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9., *Draberova E, Draber P, Havlicek F, Viklicky V: A common antigenic determinant of vimentin and desmin defined by monoclonal antibody. Folia Biol (Praha). 1986;32(5):295-303.
  • Gene target
    Vimentin monoclonal
  • isotype filter
    • IgM
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Alternative technique
    antibodies