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Syk monoclonal antibody

  • Catalog number
    11-376-C100
  • Product name
    Syk monoclonal antibody
  • Size
    100ug
  • Short name
    Anti-Syk Mab
  • Long name
    Mouse Monoclonal Antibody against Mouse Syk, Clone: SYK-01
  • Antigen
    Syk
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Mouse (Mus musculus), Human (Homo sapiens), Rat, Other not tested
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    SYK-01
  • Isotype
    IgG1
  • Applications
    IP, WB, IHC(P), ICC
  • Immunogen
    Recombinant fragment (aa 5-360) of human Syk.
  • Format conjugation
    purified; unconjugated
  • Specificity
    The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
  • Research area
    Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal Antibody against Mouse Syk, Clone: SYK-01 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by protein-A affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. _x000D_ , *Arudchandran R, Brown MJ, Peirce MJ, Song JS, Zhang J, Siraganian RP, Blank U, Rivera J: The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1. J Exp Med. 2000 Jan 3;191(1):47-60. _x000D_ , *Halova I, Draberova L, Draber P: A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor. Int Immunol. 2002 Feb;14(2):213-23. _x000D_
  • Alternative to gene target
    • [ "spleen tyrosine kinase", "p72-Syk", "SYK and IDBG-75571 and ENSG00000165025 and 6850", "transferase activity", "Plasma membranes", "Syk and IDBG-152695 and ENSMUSG00000021457 and 20963", "SYK and IDBG-637026 and ENSBTAG00000004767 and 515515" ]
  • Gene target
    Syk monoclonal
  • isotype filter
    • IgG1
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Alternative technique
    antibodies