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Mouse IgG2b Isotype Control

  • Catalog number
    1P-692-C100
  • Product name
    Mouse IgG2b Isotype Control
  • Size
    100ug
  • Short name
    IgG2b Isotype Control
  • Long name
    Mouse Monoclonal IgG2b Isotype Control
  • Antigen
    Mouse IgG2b Isotype Control
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Immunoglobulin Isotype Control
  • Negative species reactivity
    Human, Mouse, Rat, Other species Not tested
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    MPC-11
  • Isotype
    IgG2b, IgG2b
  • Applications
    FC, IP, WB, IHC(P), IHC(F), ICC, negative control
  • Immunogen
    KLH-coupled trinitrophenol
  • Format conjugation
    PE
  • Specificity
    This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
  • Research area
    Immunoglobulin Isotype Controls
  • Concentration
    0.1 mg/ml
  • Storage buffer
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
  • Shipping and storage
    Mouse Monoclonal IgG2b Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
  • Background
    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • Datasheet
    Datasheet
  • References
    *And other., *Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24., *Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95., *Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20., *Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. , *Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35.
  • Gene target
    IgG2b
  • isotype filter
    • IgG2b
  • technique filter
    • Control
    • Isotype
    • Mouse
  • Technique
    Control, Isotype, Mouse, controls, Isotypes and controls, mouses
  • Alternative technique
    control, controls, murine
  • Tissue
    control
  • tissue filter
    • control