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Mouse IgG2a Isotype Control

  • Catalog number
    1B-724-C100
  • Product name
    Mouse IgG2a Isotype Control
  • Size
    100ug
  • Short name
    IgG2a Isotype Control
  • Long name
    Mouse Monoclonal IgG2a Isotype Control
  • Antigen
    Mouse IgG2a Isotype Control
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Immunoglobulin Isotype Control
  • Negative species reactivity
    Human, Mouse, Rat
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    MOPC-173
  • Isotype
    IgG2a, IgG2a
  • Applications
    FC, IP, WB, IHC, ICC, negative control
  • Immunogen
    The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
  • Format conjugation
    biotin
  • Specificity
    This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
  • Research area
    Immunoglobulin Isotype Controls
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal IgG2a Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
  • Background
    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61., *Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20., *Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74., *Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31., *And many other.
  • Gene target
    IgG2a
  • isotype filter
    • IgG2a
  • technique filter
    • Control
    • Isotype
    • Mouse
  • Technique
    Control, Isotype, Mouse, controls, Isotypes and controls, mouses
  • Alternative technique
    control, controls, murine
  • Tissue
    control
  • tissue filter
    • control