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Mouse IgG1 Isotype Control

  • Catalog number
    1B-632-C025
  • Product name
    Mouse IgG1 Isotype Control
  • Size
    25ug
  • Short name
    IgG1 Isotype Control
  • Long name
    Mouse Monoclonal IgG1 Isotype Control
  • Antigen
    Mouse IgG1 Isotype Control
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Immunoglobulin Isotype Control
  • Negative species reactivity
    Human, Rat
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    MOPC-21
  • Isotype
    IgG1, IgG1
  • Applications
    FC, IP, WB, IHC(P), IHC(F), negative control
  • Immunogen
    Ig Control
  • Format conjugation
    biotin
  • Specificity
    This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
  • Research area
    Immunoglobulin Isotype Controls
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal IgG1 Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
  • Background
    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936., *Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12., *Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35., *Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99., *Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46., *Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
  • Gene target
    IgG1
  • isotype filter
    • IgG1
  • technique filter
    • Control
    • Isotype
    • Mouse
  • Technique
    Control, Isotype, Mouse, controls, Isotypes and controls, mouses
  • Alternative technique
    control, controls, murine
  • Tissue
    control
  • tissue filter
    • control