gentaur logogentaur logo
x

Lyn monoclonal antibody

  • Catalog number
    11-377-C025
  • Product name
    Lyn monoclonal antibody
  • Size
    25ug
  • Short name
    Anti-Lyn Mab
  • Long name
    Mouse Monoclonal Antibody against Mouse Lyn, Clone: LYN-01
  • Antigen
    Lyn
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Mouse (Mus musculus), Human (Homo sapiens), Rat
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    LYN-01
  • Isotype
    IgG1
  • Applications
    IP, WB, ICC
  • Immunogen
    Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).
  • Format conjugation
    purified; unconjugated
  • Specificity
    The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
  • Research area
    Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphorylation (Kinases, Phosphatases, Phosphotyrosine)
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal Antibody against Mouse Lyn, Clone: LYN-01 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by protein-A affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P.: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21(24):8318-28. _x000D_ , *Amoui M, Draberova L, Tolar P, Draber P.: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27(1):321-8. _x000D_ , *Draberova L, Amoui M, Draber P.: Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane_x000D_ microdomains. Immunology. 1996 Jan;87(1):141-8. _x000D_
  • Gene target
    Lyn monoclonal
  • isotype filter
    • IgG1
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Alternative technique
    antibodies