CD8a monoclonal antibody

• Catalog number
  11-678-C025
• Price
  Please ask
• Size
  25ug

• Long name
  Mouse Monoclonal Antibody against Rat CD8a, Clone: OX-8
• Antigen
  CD8a (rat)
• Species reactivity
  Rat
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  OX-8
• Applications
  FC, IP, WB, IHC(P), IHC(F), FUNC
• Immunogen
  High Mw glycoproteins from rat thymocytes
• Format conjugation
  purified; unconjugated
• Specificity
  The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
• Research area
  CD and Related Antigens & MHC (Rodent)
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Mouse Monoclonal Antibody against Rat CD8a, Clone: OX-8 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  *Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 2009;4(5):e5468., *Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15;179(6):3772-9., *Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is_x000D_ required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23;4(11):e7840., *Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not_x000D_ physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4;198(3):483-9., *Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15;160(2):700-7., *Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40., *Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8., *Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593-600.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD8a   monoclonal  
• Short name
  Anti-CD8a Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD8a (rat)
• Alternative technique
  antibodies
• Alternative to gene target
  CD8a molecule, CD8 and Leu2 and MAL and p32, CD8A and IDBG-59987 and ENSG00000153563 and 925, protein homodimerization activity, Extracellular, Cd8a and IDBG-155582 and ENSMUSG00000053977 and 12525, CD8A and IDBG-634911 and ENSBTAG00000021141 and 281060

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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