- Catalog numberPB-207-T025
- Product nameCD8 monoclonal antibody
- Size25 assays
- PriceAsk For Price
- Short nameAnti-CD8 Mab
- Long nameMouse Monoclonal Antibody against Human CD8, Clone: MEM-31
- AntigenCD8
- HostMouse (Mus musculus)
- Species reactivityHuman (Homo sapiens)
- Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
- ClonalityMonoclonal Antibody (Mab)
- CloneMEM-31
- IsotypeIgG2a
- ApplicationsFC, IP, MC
- ImmunogenCrude thymus membrane fraction.
- Format conjugationPacific Blue
- SpecificityThe antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.HLDA III; WS Code T 575
- Research areaCD and Related Antigens (Human)
- Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
- Shipping and storageMouse Monoclonal Antibody against Human CD8, Clone: MEM-31 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
- BackgroundThe CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
- Purity> 95% (by SDS-PAGE)
- Purification methodPurified by affinity chromatography
- Datasheet
- References*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., *Horejsi V et al.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen._x000D_ Folia Biol. (Praha) 32, 12 (1986). , *Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by_x000D_ the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451., *Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178(7):4402-10., *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62., *Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel_x000D_ broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34._x000D_ _x000D_ _x000D_ , *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
- Gene targetCD8 monoclonal
- Identity:HGNC:1706
- Gene:CD8A
- Long gene name:CD8a molecule
- Synonyms gene name:CD8 antigen, alpha polypeptide (p32), T-cell surface glycoprotein CD8 alpha chain
- Discovery year:1986-01-01
- Entrez gene record:925
- Pubmed identification:1541829
- RefSeq identity:NM_001768
- Classification:V-set domain containing, CD molecules
- VEGA ID:OTTHUMG00000130265
- Gene symbolCD8A
- isotype filter
- IgG2a
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- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
- Alternative techniqueantibodies
Gene info
Locus Specific Databases:CD8Abase: Mutation registry for CD8 deficiencyLRG_44