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CD45 monoclonal antibody

  • Catalog number
    1A-553-C025
  • Product name
    CD45 monoclonal antibody
  • Size
    25ug
  • Short name
    Anti-CD45 Mab
  • Long name
    Rat Monoclonal Antibody against Mouse CD45, Clone: EM-05
  • Antigen
    CD45 (mouse)
  • Host
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus)
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    EM-05
  • Isotype
    IgG
  • Applications
    FC, IP, WB, ICC
  • Immunogen
    Murine peripheral blood leukocytes_x000D_
  • Format conjugation
    APC
  • Specificity
    The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
  • Research area
    Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Rodent), CD and Related Antigens & MHC (Rodent)
  • Concentration
    0.5 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Rat Monoclonal Antibody against Mouse CD45, Clone: EM-05 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Shen KY, Song YC, Chen IH, Chong P, Liu SJ: Depletion of tumor-associated macrophages enhances the anti-tumor immunity induced by a Toll-like receptor agonist-conjugated peptide. Hum Vaccin Immunother. 2014;10(11):3241-50.
  • Gene target
    CD45 monoclonal
    • Gene info
    Gene info
    • Identity:HGNC:9666
    • Gene:PTPRC
    • Long gene name:protein tyrosine phosphatase receptor type C
    • Synonyms:LCAT200GP180
    • Discovery year:1986-01-01
    • Entrez gene record:5788
    • Pubmed identification:2169617
    • RefSeq identity:NM_002838
    • Classification:Protein tyrosine phosphatases receptor type, Fibronectin type III domain containing, CD molecules
    • VEGA ID:OTTHUMG00000035702
    Locus Specific Databases:PTPRCbase: Mutation registry for CD45 deficiencyLRG_95
  • Gene symbol
    PTPRCAP, PTPRC
  • isotype filter
    • IgG
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Alternative technique
    antibodies