NM LYSE: Flow Cytometry Lysing Solution, Other Reagents

• Catalog number
  GAS-003
• Price
  Please ask
• Size
  300Tests

• Host Source
  NA
• Clone Name
  NA
• Product Category
  NA
• Species Reactivity
  NA
• Application
  Flow Cytometry
• Immunogen
  NA
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  NM LYSE: Flow Cytometry Lysing Solution, Other Reagents reagent should be stored and used at room temperature. _x000B_Stability of the reagent: Please refer to the expiry date printed onto _x000B_the vial. The use of the reagent after the expiration date is not _x000B_recommended. Do not use reagent if a precipitate should form or _x000B_discoloration occurs. _x000B_If unexpected results are obtained which cannot be attributed to _x000B_differences in laboratory procedures, please contact us
• Datasheet link
  NA
• Description
  FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  LYSE   Lysing   Other   Reagents  
• Short name
  NM LYSE: Lysing , Other Reagents
• Technique
  Flow Cytometry, Flow
• Isotype
  NA
• Alternative name
  nanometer LYSE: Flow Cytometry Lysing solution, Other Reagents

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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