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Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit

  • Catalog number
    ELK7717
  • Product name
    Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit
  • Size
    96T
  • Alternative Names
    CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
  • Assay Type
    Competitive Inhibition
  • Sensitivity
    51.7pg/mL
  • Standard
    10000pg/mL
  • Detection range
    123.5-10000pg/mL
  • Assay length
    2h
  • Research Area
    Neuro science;
  • Test principle
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurofilament, Light Polypeptide (NEFL) and unlabeled Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample.
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "neurofilament, light polypeptide", "CMT1F and CMT2E and NF-L and NF68 and NFL and PPP1R110", "NEFL and IDBG-777808 and ENSG00000277586 and 4747", "protein binding", "Cytoplasm", "Nefl and IDBG-176493 and ENSMUSG00000022055 and 18039", "NEFL and IDBG-635366 and ENSBTAG00000021949 and 281348" ]
  • Gene target
    NEFL Neurofilament Light Polypeptide
    • Gene info
    Locus Specific Databases:Inherited Peripheral Neuropathies Mutation DatabaseLRG_259
  • Gene symbol
    NEFL
  • Host
    Rat, antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    NEFL(Neurofilament, Light Polypeptide) ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
    • light
  • Technique
    ELISA kit, ELISA, light, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits