- Catalog numberELK2292
- Product nameHuman MYLK(Myosin Light Chain Kinase) ELISA Kit
- Size96T
- PriceAsk For Price
- Alternative NamesKRP; MLCK108; MLCK210; MSTP083; MLCK; smMLCK; MYLK1; MLCK1; Telokin; Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form; Kinase-related protein
- Assay TypeSandwich
- Sensitivity0.064ng/mL
- Standard10ng/mL
- Detection range0.156-10ng/mL
- Assay length3h
- Research AreaEnzyme & Kinase;Cardiovascular biology;
- Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain Kinase (MYLK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain Kinase (MYLK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain Kinase (MYLK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain Kinase (MYLK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetMYLK Myosin Light Chain Kinase
- Identity:HGNC:42440
- Gene:MYLK-AS1
- Long gene name:MYLK antisense RNA 1
- Synonyms gene name:MYLK antisense RNA 1 (non-protein coding)
- Discovery year:2011-07-27
- Entrez gene record:100506826
- Classification:Antisense RNAs
- VEGA ID:OTTHUMG00000159536
- Identity:HGNC:40387
- Gene:MYLK-AS2
- Long gene name:MYLK antisense RNA 2
- Synonyms gene name:MYLK antisense RNA 2 (non-protein coding)
- Discovery year:2011-07-27
- Entrez gene record:100873940
- Classification:Antisense RNAs
- VEGA ID:OTTHUMG00000159965
- Identity:HGNC:7590
- Gene:MYLK
- Long gene name:myosin light chain kinase
- Synonyms gene name:myosin, light polypeptide kinase
- Synonyms:MLCKsmMLCKMYLK1MLCK1
- Discovery year:1995-07-14
- Entrez gene record:4638
- Pubmed identification:8575746
- RefSeq identity:NM_053025
- Classification:Fibronectin type III domain containing, Myosin light chain kinase family, I-set domain containing
- VEGA ID:OTTHUMG00000141304
- Gene symbolMYLK-AS1, MYLK-AS2, MYLK
- Hostantigen assay ELISA kit 1 Kit (96 Wells)
- Short nameMYLK(Myosin Light Chain Kinase) ELISA Kit
- technique filter
- ELISA kit
- ELISA
- light
- TechniqueELISA kit, ELISA, light, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
- Alternative techniqueelisas, kits