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Human C5a(Complement Component 5a) ELISA Kit

  • Catalog number
    ELK1051
  • Product name
    Human C5a(Complement Component 5a) ELISA Kit
  • Size
    96T
  • Assay Type
    Sandwich
  • Sensitivity
    27pg/mL
  • Standard
    5000pg/mL
  • Detection range
    78-5000pg/mL
  • Assay length
    3h
  • Research Area
    Infection immunity;Immune molecule;Autoimmunity;
  • Test principle
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a (C5a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5a (C5a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5a (C5a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5a (C5a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    C5a Complement Component 5a
    • Gene info
    Gene info
  • Gene symbol
    C5AR1, C5AR2
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    C5a(Complement Component 5a) ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits