TT Cell Line

• Catalog number
  EP-CL-0457
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  TT
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  77 years
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  TT cells continuously produce high levels of calcitonin and CEA.Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively, after a medium change.CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period.Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1mM L-glutamine.It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F-12K medium.Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes.
• Biosafety
  1
• Medium
  Ham's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
• Receptor_expression
  NA
• Antigen_expression
  NA
• Gene_expression
  Calcitonin; Carcinoembryonic Antigen(Cea)
• Applications
  NA
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Line  
• Short name
  TT Line
• Species
  Homo sapiens, Human
• Alternative name
  TT cellular Line
• Tissue
  Thyroid/medulla

Similar products