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RF/6A Cell Line

  • Catalog number
    EP-CL-0194
  • Product name
    RF/6A Cell Line
  • Size
    1×10⁶ cells/vial
  • Price
    Ask For Price
  • Contact us
    RF/6A Cell Line
  • Abbreviation
    RF/6A
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    Fetus
  • Tissue
    Choroid, retina
  • Morphology
    Epithelial
  • Growth properties
    Adherent
  • Doubling time
    NA
  • Background
    Weibel-Palade granules are seen by electron microscopy.
  • Biosafety
    1
  • Medium
    MEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    NA
  • Effects
    NA
  • Receptor expression
    NA
  • Antigen expression
    NA
  • Gene expression
    Factor Ⅷ; Fibronectin
  • Applications
    Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
  • Duration
    NA
  • Technical data
    Technical_data
  • Gene target
    RF/6A Line
  • Short name
    RF/6A Line
  • tissue filter
    • Choroid
    • retina