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Recombinant Mouse Acetylcholinesterase / ACHE Protein (His tag) 20µg

  • Catalog number
    PKSM040622-20µg
  • Product name
    Recombinant Mouse Acetylcholinesterase / ACHE Protein (His tag) 20µg
  • Size
    20µg
  • Activity
    Measured by its ability to cleave Acetylthiocholine.The specific activity is > 250 nmols/min/μg.
  • Synonym
    mE1a, mE1b, mE1c, mE1c-long, mE1d, mE1d', mE1e
  • Sequence
    Met 1-Leu 614
  • Fusion tag
    C-His
  • Accession
    NP_033729.1
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    > 97 % as determined by SDS-PAGE
  • Endotoxin
    < 1.0 EU per μg of the protein as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    66.2 kDa
  • AP Mol Mass
    66.2 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.4
  • Background
    Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.
  • Technical data
    Technical_data
  • Alternative to gene target
    • [ "acetylcholinesterase", "ACEE and ARACHE and N-ACHE and YT", "ACHE and IDBG-32513 and ENSG00000087085 and 43", "protein self-association", "nuclei", "Ache and IDBG-204838 and ENSMUSG00000023328 and 11423", "ACHE and IDBG-640435 and ENSBTAG00000001139 and 540446" ]
  • Gene target
    Acetylcholinesterase / ACHE Protein His tag 20µg
    • Gene info
    Locus Specific Databases:Blood Group Antigen Mutation DatabaseLRG_804
  • Gene symbol
    ACHE
  • Host
    mouse
  • Short name
    Recombinant Mouse Acetylcholinesterase / ACHE Protein (His tag) 20µg
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
    • Mouse
  • Technique
    Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec, murine