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Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (His & Fc tag)

  • Catalog number
    PKSH031622-100µg
  • Product name
    Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (His & Fc tag)
  • Size
    100µg
  • Synonym
    CD264;DCR2;TRAIL-R4;TRAILR4;TRUNDD
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10D Fc Chimera at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.625-20 ng/ml.2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
  • Sequence
    Met 1-His 211
  • Fusion tag
    C-His & Fc
  • Accession
    NP_003831.2
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    ＞98 % as determined by SDS-PAGE
  • Endotoxin
    ＜1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    44.7 kDa
  • AP Mol Mass
    65-80 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.4
  • Background
    Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-κB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.
  • Technical data
    Technical_data
  • Alternative to gene target
    • [ "tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain", "CD264 and DCR2 and TRAIL-R4 and TRAILR4 and TRUNDD", "TNFRSF10D and IDBG-11632 and ENSG00000173530 and 8793", "TRAIL binding", "Plasma membranes" ]
  • Gene target
    TRAIL R4 / CD264 / TNFRSF10D Protein His Fc tag
    • Gene info
    Gene info
    Gene info
  • Gene symbol
    TNFRSF10D, TNFSF10, TNFRSF10B
  • Short name
    Recombinant TRAIL R4 / CD264 / TNFRSF10D Protein (His & Fc tag)
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
    • FC
  • Technique
    Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec