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Recombinant Human PRKD2 / PKD2 Protein (His & GST tag)

  • Catalog number
    PKSH030353-20µg
  • Product name
    Recombinant Human PRKD2 / PKD2 Protein (His & GST tag)
  • Size
    20µg
  • Synonym
    HSPC187;nPKC-D2;PKD2
  • Activity
    The specific activity was determined to be ＞30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
  • Sequence
    Met 1-Leu 878
  • Fusion tag
    N-His & GST
  • Accession
    NP_057541.2
  • Expressed Host
    Baculovirus-Insect Cells
  • Shipping
    Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
  • Purity
    ＞82 % as determined by SDS-PAGE
  • Endotoxin
    ＜1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    124 kDa
  • AP Mol Mass
    120 kDa
  • Formulation
    Supplied as sterile 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0
  • Background
    Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • Technical data
    Technical_data
  • Alternative to gene target
    • [ "protein kinase D2", "nPKC-D2 and PKD2", "PRKD2 and IDBG-58915 and ENSG00000105287 and 25865", "transferase activity", "nuclei", "Prkd2 and IDBG-147053 and ENSMUSG00000041187 and 101540", "PRKD2 and IDBG-639857 and ENSBTAG00000015599 and 782793" ]
  • Gene target
    PRKD2 / PKD2 Protein His GST tag
    • Gene info
    Gene info
    Gene info
    Locus Specific Databases:Global Variome shared LOVD
  • Gene symbol
    PRKD2, MGST3, PKD2
  • Short name
    Recombinant PRKD2 / PKD2 Protein (His & GST tag)
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec