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Recombinant Human GSK3B Protein (His tag)

  • Catalog number
    PKSH030425-20µg
  • Product name
    Recombinant Human GSK3B Protein (His tag)
  • Size
    20µg
  • Synonym
    GSK3B
  • Activity
    The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
  • Sequence
    Met 1-Thr 433
  • Fusion tag
    N-His
  • Accession
    NP_002084.2
  • Expressed Host
    Baculovirus-Insect Cells
  • Shipping
    Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
  • Purity
    ＞90 % as determined by SDS-PAGE
  • Endotoxin
    ＜1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    50.4 kDa
  • AP Mol Mass
    44-48 kDa
  • Formulation
    Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA
  • Background
    GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.
  • Technical data
    Technical_data
  • Alternative to gene target
    • [ "glycogen synthase kinase 3 beta", "GSK3B and IDBG-51663 and ENSG00000082701 and 2932", "transferase activity", "nuclei", "Gsk3b and IDBG-159859 and ENSMUSG00000022812 and 56637", "BT.48740 and IDBG-632831 and ENSBTAG00000048057 and 790875" ]
  • Gene target
    GSK3B Protein His tag
    • Gene info
    Gene info
    • Identity:HGNC:55635
    • Gene:GSK3B-DT
    • Long gene name:GSK3B divergent transcript
    • Discovery year:2021-06-24
    • Entrez gene record:107986119
    • Classification:Divergent transcripts
  • Gene symbol
    GSK3B, GSK3B-DT
  • Short name
    Recombinant GSK3B Protein (His tag)
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec