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Recombinant Human CD86 / B7-2 Protein (His & Fc tag)

  • Catalog number
    PKSH031475-100µg
  • Product name
    Recombinant Human CD86 / B7-2 Protein (His & Fc tag)
  • Size
    100µg
  • Synonym
    B7-2;B7.2;B70;CD28LG2;LAB72
  • Activity
    Measured by its ability to bind human CD28 in a functional ELISA.2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.The ED50 for this effect is 1-5μg/mL.
  • Sequence
    Met 1-His 239
  • Fusion tag
    C-His & Fc
  • Accession
    NP_008820.2
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    ＞95 % as determined by SDS-PAGE
  • Endotoxin
    ＜1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    53.2 kDa
  • AP Mol Mass
    80-90 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.4
  • Background
    CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy
  • Technical data
    Technical_data
  • Alternative to gene target
    • [ "CD86 molecule", "B7-2 and B7.2 and B70 and CD28LG2 and LAB72", "CD86 and IDBG-52336 and ENSG00000114013 and 942", "coreceptor activity", "Cell surfaces", "Cd86 and IDBG-158071 and ENSMUSG00000022901 and 12524", "CD86 and IDBG-633264 and ENSBTAG00000013118 and 414345" ]
  • Gene target
    CD86 / B7-2 Protein His Fc tag
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    CD86, CD276, HHLA2, RNY4P2, LRRC23, PCDHGB7, NCR3LG1, CD274, TLX1NB, CD80
  • Short name
    Recombinant CD86 / B7-2 Protein (His & Fc tag)
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
    • FC
  • Technique
    Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec