Recombinant Human BACE1 / ASP2 Protein (Fc tag)
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Catalog number
PKSH031902-50µg
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Price
Please ask
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Size
50µg
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Synonym
ASP2;BACE;HSPC104
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Activity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2, (R&D Systems, Catalog # ES004). The specific activity is >0.5 pmoles/min/μg.
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Sequence
Met 1-Thr 457
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Fusion tag
C-Fc
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Accession
NP_036236.1
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
>90 % as determined by SDS-PAGE
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Endotoxin
<1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
75 kDa
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AP Mol Mass
100-105 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
BACE1, BACE1-AS
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Short name
Recombinant BACE1 / ASP2 Protein (Fc tag)
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Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
Fc Tag
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Species
Human, Humans
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Alternative name
Rec. H. sapiens BACE1 / ASP2 Protein (fragment c detection labelled)
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
beta-secretase 1
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Synonyms gene
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Synonyms gene name
- beta-site APP-cleaving enzyme
- beta-site APP-cleaving enzyme 1
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GenBank acession
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Locus
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Discovery year
1999-10-29
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
BACE1 antisense RNA
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Synonyms gene
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Synonyms gene name
- BACE1 antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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Locus
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Discovery year
2009-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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