RAW 264.7 Cell Line

  • Catalog number
    EP-CL-0190
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    RAW 264.7
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    Adult
  • Morphology
    Monocyte/macrophage
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin(sIg-), Ia(Ia-) and Thy-1.2(Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.Ref
  • Biosafety
    2
  • Medium
    DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
  • Tumorigenic
    NA
  • Effects
    NA
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    NA
  • Applications
    This cell line is a suitable transfection host. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
    RAW   264   Line  
  • Short name
    RAW 264 7 Line
  • Species
    Mus musculus, Mouse
  • Alternative name
    RAW 264.7 cellular Line
  • Tissue
    Abelson murine leukemia virus-induced tumor, ascites
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