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Abbreviation
RAW 264.7
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
Adult
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Morphology
Monocyte/macrophage
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Growth_properties
Adherent
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Doubling_time
NA
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Background
This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin(sIg-), Ia(Ia-) and Thy-1.2(Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.Ref
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Biosafety
2
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Medium
DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Subcultures are prepared by scraping or blowing. For a 100mm disk,remove all but 10 mL culture medium (adjust amount accordingly for otherculture vessels). Dislodge cells from the flask substrate with a cell scraperor blowing directly with the pipettes; aspirate and add appropriatealiquots of the cell suspension into new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
This cell line is a suitable transfection host. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.