Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit

• Catalog number
  E-EL-R1436-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Rat
• Target Synonym
  PACAP-27
• Test principle
  This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Rat PACAP-27. During the reaction, Rat PACAP-27 in the sample or standard competes with a fixed amount of Rat PACAP-27 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-27. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sensitivity
  4.69pg/mL
• Detection Range
  7.81~500pg/mL
• Target Name
  PACAP-27
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit
• Type
  Competitive
• Technical data
• Standard Curve RUL
• Description
  This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  PACAP-27   Pituitary   Adenylate   Cyclase   Activating   Polypeptide   Kit  
• Gene symbol
  ADCYAP1, SNORD115-27, IGKV1D-27, ERVK-27, ERVW-27, RNVU1-27, IGHV7-27, SNORD116-27, SNORD114-27, IGLV3-27
• Short name
  PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) EIA Kit
• Technique
  EIA, eia
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat PACAP-27(Pituitary Adenylate Cyclase Activating multipeptide 27) EIA reagent
• Alternative technique
  kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:241
• Gene
  ADCYAP1
• Long gene name
  adenylate cyclase activating polypeptide 1
• Synonyms gene name
  • adenylate cyclase activating polypeptide 1 (pituitary)
• Synonyms
  • PACAP
• Synonyms name
  • prepro-PACAP
  • pituitary adenylate cyclase activating polypeptide
• GenBank acession
  • S83513
• Locus
  18p11.32
• Discovery year
  1992-12-02
• Entrez gene record
  116
• Pubmed identfication
  • 1730060
• RefSeq identity
  • NM_001117
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000131479

Gene info

• Identity
  HGNC:33046
• Gene
  SNORD115-27
• Long gene name
  small nucleolar RNA, C/D box 115-27
• Synonyms
  • HBII-52-27
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100036564
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003496
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5751
• Gene
  IGKV1D-27
• Long gene name
  immunoglobulin kappa variable 1D-27 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1D-27
• GenBank acession
  • Z00004
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28898
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151607

Gene info

• Identity
  HGNC:44980
• Gene
  ERVK-27
• Long gene name
  endogenous retrovirus group K member 27
• Synonyms gene name
  • endogenous retrovirus group K, member 27
• GenBank acession
  • JZ164909
• Locus
  11q12.3
• Discovery year
  2014-01-03
• Pubmed identfication
  • 23338945

Gene info

• Identity
  HGNC:49315
• Gene
  ERVW-27
• Long gene name
  endogenous retrovirus group W member 27
• Synonyms gene name
  • endogenous retrovirus group W, member 27
• GenBank acession
  • HG421064
• Locus
  14q32.12
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:54431
• Gene
  RNVU1-27
• Long gene name
  RNA, variant U1 small nuclear 27
• Locus
  1q21.2
• Discovery year
  2019-07-26
• Classification
  • Variant U1 small nuclear RNAs

Gene info

• Identity
  HGNC:5663
• Gene
  IGHV7-27
• Long gene name
  immunoglobulin heavy variable 7-27 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 7-27
  • immunoglobulin heavy variable 7-27 pseudogene
• GenBank acession
  • M99643
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28383
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152070

Gene info

• Identity
  HGNC:33093
• Gene
  SNORD116-27
• Long gene name
  small nucleolar RNA, C/D box 116-27
• Synonyms
  • HBII-85-27
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033439
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003341
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33015
• Gene
  SNORD114-27
• Long gene name
  small nucleolar RNA, C/D box 114-27
• Synonyms
  • 14q(II-27)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767608
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003220.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5910
• Gene
  IGLV3-27
• Long gene name
  immunoglobulin lambda variable 3-27
• GenBank acession
  • D86994
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28791
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151171

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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