- Catalog numberEP-CL-0158
- Product nameMGC80-3 Cell Line
- Size1×10⁶ cells/vial
- PriceAsk For Price
- AbbreviationMGC80-3
- Leadtime3-5 days
- Shipping conditionDry ice
- StorageLiquid nitrogen -196°C
- AgeNA
- TissueStomach
- MorphologyEpithelial
- Growth propertiesAdherent
- Doubling timeNA
- BackgroundNA
- Biosafety1
- MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
- SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
- Ratio1:2-1:4
- RenewalEvery 2 to 3 days
- CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
- Culture conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
- TumorigenicYes
- EffectsYes, forms tumors in nude mice.
- Receptor expressionNA
- Antigen expressionNA
- Gene expressionNA
- ApplicationsNA
- DurationNA
- Technical data
- Gene targetMGC80-3 Line
- Identity:HGNC:35295
- Gene:MIR1302-3
- Long gene name:microRNA 1302-3
- Synonyms:hsa-mir-1302-3
- Discovery year:2008-11-03
- Entrez gene record:100302128
- RefSeq identity:NR_031632
- Classification:MicroRNA MIR1302 family
- Identity:HGNC:33675
- Gene:MIR509-3
- Long gene name:microRNA 509-3
- Synonyms:hsa-mir-509-3
- Discovery year:2007-10-24
- Entrez gene record:100126337
- RefSeq identity:NR_030629
- Classification:MicroRNA MIR506 family
- Identity:HGNC:31640
- Gene:MIR7-3
- Long gene name:microRNA 7-3
- Synonyms:hsa-mir-7-3
- Discovery year:2004-04-23
- Entrez gene record:407045
- RefSeq identity:NR_029607
- Classification:MicroRNA MIR7 family
- Identity:HGNC:37517
- Gene:PIRC13
- Long gene name:piwi-interacting RNA cluster 13
- Discovery year:2009-11-05
- Entrez gene record:100313925
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Identity:HGNC:37515
- Gene:PIRC11
- Long gene name:piwi-interacting RNA cluster 11
- Discovery year:2009-11-05
- Entrez gene record:100313897
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Identity:HGNC:37518
- Gene:PIRC14
- Long gene name:piwi-interacting RNA cluster 14
- Discovery year:2009-11-05
- Entrez gene record:100313784
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Identity:HGNC:37516
- Gene:PIRC12
- Long gene name:piwi-interacting RNA cluster 12
- Discovery year:2009-11-05
- Entrez gene record:100313808
- Pubmed identification:17881367
- Classification:Piwi-interacting RNA clusters
- Gene symbolMIR1302-3, MIR509-3, MIR7-3, PIRC13, PIRC11, PIRC14, PIRC12
- Short nameMGC80-3 Line
- tissue filter
- Stomach