Human ADAM10(A Disintegrin And Metalloprotease 10) EIA Kit
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Catalog number
E-EL-H0263-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
AD10, CD156c, CDw156, HsT18717, MADM, kuz, ADAM metallopeptidase domain 10
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM10. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADAM10. You can calculate the concentration of Human ADAM10 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
37.5pg/mL
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Detection Range
62.5~4000pg/mL
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Target Name
ADAM10
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UNIProt ID
O14672
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human ADAM10(A Disintegrin And Metalloprotease 10) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbol
ADAM10
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Short name
ADAM10(A Disintegrin Metalloprotease 10) EIA Kit
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Technique
EIA, eia
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Species
Human, Humans
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Alternative name
H. sapiens ADAM metallopeptidase domain 10(A Disintegrin And Metalloprotease 10) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
ADAM metallopeptidase domain 10, AD10 and AD18 and CD156c and HsT18717 and kuz and MADM and RAK, ADAM10 and IDBG-14155 and ENSG00000137845 and 102, protein homodimerization activity, nuclei, Adam10 and IDBG-182594 and ENSMUSG00000054693 and 11487, ADAM10 and IDBG-646594 and ENSBTAG00000005481 and 282132
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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