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Recombinant Vascular Endothelial Growth Factor C (VEGFC)

  • Catalog number
    RAM/A2M
  • Product name
    Rabbit anti Mouse alpha-2 macroglobulin
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
  • Antibody come from
    Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Rabbit anti Mouse alpha-2 macroglobulin Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: Bovine ± ferret ± Horse ± Rat ++ Cat ± Goat - Human -Sheep - Chicken - Guinea Pig ± Monkey - Swine ± Dog - Hamster + pigeon - Turkey -
  • Label
    unlabeled
  • Antibody s specificity
    Precipitating polyclonal Rabbit antiSerum to Mouse alpha-2 macroglobulin
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre 1:32 when tested against pooled normal mouse sera of different strains in agar-block immunodiffusion titration.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    alpha-2 macroglobulin
    • Gene info
    Gene info
    Gene info
  • Gene symbol
    A2MP1, PZP, A2ML1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti Mouse alpha-2 macroglobulin
  • label filter
    • unlabeled
  • technique filter
    • anti mouse
    • Rabbit
    • Mouse
    • anti
  • Technique
    anti mouse, Rabbit, Mouse, anti, antibody to, anti mouse antibodies, mouses
  • Alternative technique
    rabbit-anti, murine, antibodies