100ug-Anti-FLAG Octapeptide (FLAG) Tag Polyclonal Antibody
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Catalog numberTAX159Ge01-100ug
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Price:
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Size100ug
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Organism SpeciesPan-species (General)
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SourcePolyclonal antibody preparation
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PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
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Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Item NameFLAG Octapeptide
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ImmunogenCPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
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Image number-
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Species reactivityPan-species
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Sequence of immunogenCPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
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AplicationWB; IHC; ICC; IP.
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ClonalityRabbit polyclonal
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Concentration500ug/ml
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Alternative NamesFLAG epitope; FLAG-Tag
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Applicable Secondary AntibodySAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
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Delivery condition4℃ with ice bags
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Storage instructionsAvoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Short name100ug-Anti- Octapeptide ( ) Tag Polyclonal Antibody
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TechniquePolyclonal, Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
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HostRabbit
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LabelFlag
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Alternative name100ug-Antibody toFLAG Octapeptide (FLAG) detection labelled polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data