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S-Adenosylhomocysteine (SAH) ELISA test kit

  • Catalog number
    STA-671
  • Product name
    S-Adenosylhomocysteine (SAH) ELISA test kit
  • Size
    96 assays
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    Upon receipt, store SAH Conjugate and SAH-BSA Standard at -80ºC. Store the rest of the test kit at 4ºC.
  • Type of reagent
    Metabolism Research
  • Related techniques
    Metabolism Research, Amino Acid Metabolism, S-Adenosylhomocysteine (SAH) ELISA test kit
  • Use of the reagent
    S-adenosylhomocysteine (SAH) is generated through the donation of a methyl group from S-adenosylmethionine (SAM) to various biomolecules. SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-adenosylhomocysteine (SAH) ELISA is designed for detection and quantification of SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAH antibody are added to a plate coated with a SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.
  • Cellular use
    Detection sensitivity limit of 7ng/mL SAH-BSA
  • Protocol information
    Suitable for use with plasma, serum, lysates, and other biological fluid samples
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,56
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    S-Adenosylhomocysteine SAH kit
    • Gene info
  • Gene symbol
    ACSM3
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    S-Adenosylhomocysteine (SAH) ELISA test kit
  • technique filter
    • ELISA test
    • ELISA
  • Technique
    ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Alternative technique
    elisas, tests, kits