gentaur logogentaur logo
x

Platinum HSC Retroviral Expression System, Amphotropic

  • Catalog number
    VPK-307
  • Product name
    Platinum HSC Retroviral Expression System, Amphotropic
  • Size
    1 test kit
  • We send this reagant on
    Dry Ice
  • The temperatureerature for storage is
    Store all plasmids at -20C. For best results begin culture of Platinum cells upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
  • Type of reagent
    Viral Expression
  • Related techniques
    Stem Cell Research, Retroviral Expression Systems for Stem Cells
  • Use of the reagent
    Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-A Packaging cell line, which contains the gag and pol genes and an amphotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.
  • Cellular use
    Achieve average titer of 10^7 infectious units/mL with transient transfection
  • Protocol information
    Produce amphotropic retrovirus which will infect human, mouse and rat cells
  • Reagent properties
    Optimized systems for expression in HSC cells
  • Additional lab advice
    Cloning capacity of expression vector = 4.3 kb
  • Living modified organisme in this reagent or LMO
    Human cells transduced with retroviral genes
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,82
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Gene target
    Platinum HSC Retroviral Expression Amphotropic
    • Gene info
    Locus Specific Databases:Blood Group Antigen Mutation DatabaseLRG_810
  • Gene symbol
    FUT1
  • Short name
    Platinum HSC Retroviral Expression System, Amphotropic