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Global DNA Methylation ELISA test kit (5’-methyl-2’-deoxycytidine quantification)

  • Catalog number
    STA-380-5
  • Product name
    Global DNA Methylation ELISA test kit (5’-methyl-2’-deoxycytidine quantification)
  • Size
    5 x 96 assays
  • We send this reagant on
    Room temperature
  • The temperatureerature for storage is
    Upon receipt, aliquot and store the 5MedCyd Standard and 5MedCyd DNA Conjugate at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
  • Type of reagent
    Oxidative Stress/Damage
  • Related techniques
    Oxidative Stress/ Damage, DNA / RNA Damage and Repair, Global DNA Methylation and Hydroxymethylation
  • Use of the reagent
    The Global DNA Methylation ELISA test kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
  • Cellular use
    For detection and quantification of 5'-methyl-2'-deoxycytidine (5MedCyd)
  • Protocol information
    Suitable for use with DNA samples such as cell or tissue genomic DNA
  • Reagent properties
    Detection sensitivity of 150 nM 5MedCyd
  • Living modified organisme in this reagent or LMO
    Not applicable
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,37
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Global DNA Methylation kit 5’ methyl-2’ deoxycytidine quantification
    • Gene info
    Locus Specific Databases:LRG_162
    Gene info
    Locus Specific Databases:IMGT/HLA Database
  • Gene symbol
    PRKDC, HLA-DOA
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Global DNA ELISA test kit (5’-methyl-2’-deoxycytidine quantification)
  • technique filter
    • ELISA test
    • ELISA
    • dna
  • Technique
    ELISA test, ELISA, dna, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Alternative technique
    elisas, tests, kits