- Catalog numberVPK-411-SER2
- Product nameAAV-2 Helper Free Promoterless Expression System
- Size1 test kit
- PriceAsk For Price
- We send this reagant onBlue Ice
- The temperatureerature for storage is-20ºC
- Type of reagentViral Expression
- Related techniquesViral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-2 Helper Free Complete Expression Systems
- Use of the reagentThe AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
- Cellular useComplete Expression System contains packaging plasmids, expression vector and GFP control vector to produce recombinant AAV serotype 2
- Protocol informationpHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
- Reagent propertiesCloning capacity of expression vector = 3.9 kb
- Living modified organisme in this reagent or LMONot applicable
- Species compatible toHumans
- Use of this test kit or productGenetics lab
- Antigena specific protein sequence
- BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,08
- Molecular weightin kilo Danlto or kDa
- This reagent is purified0.97
- Gene targetAAV-2 Helper Free Promoterless Expression
- Identity:HGNC:31639
- Gene:MIR7-2
- Long gene name:microRNA 7-2
- Synonyms:hsa-mir-7-2
- Discovery year:2004-04-23
- Entrez gene record:407044
- RefSeq identity:NR_029606
- Classification:MicroRNA MIR7 family
- Identity:HGNC:32051
- Gene:MIR329-2
- Long gene name:microRNA 329-2
- Synonyms:hsa-mir-329-2
- Discovery year:2005-07-11
- Entrez gene record:574409
- RefSeq identity:NR_029968
- Classification:MicroRNA MIR329 family
- Identity:HGNC:32091
- Gene:MIR512-2
- Long gene name:microRNA 512-2
- Synonyms:hsa-mir-512-2
- Discovery year:2005-07-15
- Entrez gene record:574459
- RefSeq identity:NR_030181
- Classification:MicroRNA MIR506 family
- Identity:HGNC:32113
- Gene:MIR521-2
- Long gene name:microRNA 521-2
- Synonyms:hsa-mir-521-2
- Discovery year:2005-07-15
- Entrez gene record:574481
- RefSeq identity:NR_030203
- Classification:MicroRNA MIR515 family
- Identity:HGNC:35282
- Gene:MIR1289-2
- Long gene name:microRNA 1289-2
- Synonyms:hsa-mir-1289-2
- Discovery year:2008-11-03
- Entrez gene record:100302134
- RefSeq identity:NR_031621
- Classification:MicroRNA MIR1289 family
- Identity:HGNC:33641
- Gene:MIR509-2
- Long gene name:microRNA 509-2
- Synonyms:hsa-mir-509-2
- Discovery year:2007-10-24
- Entrez gene record:100126301
- RefSeq identity:NR_030586
- Classification:MicroRNA MIR506 family
- Identity:HGNC:18923
- Gene:KRTAP13-2
- Long gene name:keratin associated protein 13-2
- Synonyms:KAP13-2
- Discovery year:2003-02-21
- Entrez gene record:337959
- Pubmed identification:12359730
- Classification:Keratin associated proteins
- VEGA ID:OTTHUMG00000057793
- Identity:HGNC:33068
- Gene:SNORD116-2
- Long gene name:small nucleolar RNA, C/D box 116-2
- Synonyms:HBII-85-2
- Discovery year:2007-02-15
- Entrez gene record:100033414
- Pubmed identification:11106375, 11726556
- RefSeq identity:NR_003317
- Classification:Small nucleolar RNAs, C/D box
- Gene symbolAAVS1, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2
- Short nameAAV-2 Helper Free Promoterless Expression System