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CytoSelect BrdU Cell Proliferation ELISA Kit

  • Catalog number
    CBA-251
  • Product name
    CytoSelect BrdU Cell Proliferation ELISA Kit
  • Size
    96 assays
  • Shipping Temp
    Room Temp
  • Storage Temp
    Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20ºC. Store all other components at 4ºC.
  • Detection Method
    Colorimetric
  • Category Product Type
    Kit
  • URL link to datasheet
    URL_link_to_datasheet
  • URL link to SDS
    URL_link_to_SDS
  • UNSPSC
    41116158
  • Product Line
    Cell Based Assays
  • Short Description
    The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
  • Product feature 1
    Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
  • Product feature 2
    BrdU standard included
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    CytoSelect BrdU Proliferation
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    CytoSelect BrdU Proliferation ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits
  • Tissue
    cell
  • tissue filter
    • cell