- Catalog numberVPK-416-DJ-8
- Product nameAAV-DJ/8 Helper Free Bicistronic Expression System (Neo)
- Size1 kit
- PriceAsk For Price
- Shipping TempBlue Ice
- Storage Temp-20ºC
- Category Product TypeKit
- URL link to datasheet
- URL link to SDS
- UNSPSC41105908
- Product LineViral Expression
- Short DescriptionThe AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
- Product feature 1Complete Expression System contains packaging plasmids, expression vector and GFP control vector
- Product feature 2pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
- Product feature 3AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
- Gene targetAAV-DJ/8 Helper Free Bicistronic Expression Neo
- Identity:HGNC:35426
- Gene:PATE3
- Long gene name:prostate and testis expressed 3
- Synonyms:PATE-DJHEL-127
- Discovery year:2008-12-17
- Entrez gene record:100169851
- Pubmed identification:18390568
- RefSeq identity:NM_001129883
- Classification:PATE family, LY6/PLAUR domain containing
- VEGA ID:OTTHUMG00000165857
- Identity:HGNC:16369
- Gene:PARK7
- Long gene name:Parkinsonism associated deglycase
- Synonyms gene name:Parkinson disease (autosomal recessive, early onset) 7, parkinson protein 7
- Synonyms:DJ-1, DJ1GATD2
- Discovery year:2001-08-07
- Entrez gene record:11315
- Pubmed identification:11462174, 9070310, 25416785, 28596309
- RefSeq identity:NM_007262
- Classification:Glutamine amidotransferase class 1 domain containing
- VEGA ID:OTTHUMG00000001210
- Identity:HGNC:5229
- Gene:DNAJA1
- Long gene name:DnaJ heat shock protein family (Hsp40) member A1
- Synonyms gene name:DnaJ (Hsp40) homolog, subfamily A, member 1
- Synonyms:HSPF4hdj-2dj-2NEDD7
- Discovery year:1998-03-26
- Entrez gene record:3301
- Pubmed identification:8334160, 11147971
- Classification:DNAJ (HSP40) heat shock proteins
- VEGA ID:OTTHUMG00000019760
- Gene symbolAAVS1, PATE3, PARK7, DNAJA1
- Short nameAAV-DJ/8 Helper Free Bicistronic Expression System (Neo)
Gene info
Gene info
Gene info
Locus Specific Databases:Global Variome shared LOVD