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Mouse IL-3 PicoKine ELISA Kit

  • Catalog number
    EK0403
  • Product name
    Mouse IL-3 PicoKine ELISA Kit
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of mouse IL-3 in cell culture supernatant and serum.
  • Gene tested
    murine IL-3
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.013 + 0.058 + 0.095 + 0.182 + 0.296 + 0.765 + 1.474 + 2.266
  • ELISA kit detection limits
    7.8pg/ml to 500pg/ml
  • ELISA s sensitivity
    < 1pg/ml
  • Target of this ELISA
    Natural and recombinant mouse IL-3
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);32.6;156;364;43.7;164;348 ELISA standard deviations 1.793;9.516;23.66;2.75;11.81;26.1 CV(%);5.5;6.1;6.5;6.3;7.2;7.5
  • ELISA kit description
    GENTAUR's mouse IL-3 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for IL-3 has been precoated onto 96-well plates. Standards (E.coli,A27-C166) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse IL-3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse IL-3 antibodies;1 ST0000-10;Lyophilized recombinant mouse IL-3 standard;10ng/tubex2 AR1107;Biotinylated anti- mouse IL-3 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    /
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml mouse IL-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant or serum to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each mouse IL-3 standard solution and each sample is measured in duplicate.
  • Minutes of TMB incubation
    25-30
  • More gene info
    The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.
  • Antigen
    E.coli,A27-C166
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Gene symbol
    IL3
  • Uniprot
    Interleukin-3
  • Gene name
    interleukin 3
  • Alternative
    Burst promoting activity;_x000D_Colony stimulating factor multiple;_x000D_Eosinophil colony stimulating factor;_x000D__x000D__x000D_Hematopoietic growth factor;_x000D_IL 3;_x000D_IL-3;_x000D_IL3;_x000D_IL3_HUMAN;_x000D_Interleukin 3;_x000D_Interleukin-3;_x000D_Mast cell growth factor;_x000D_MCGF;_x000D_Multi CSF;_x000D_Multilineage colony stimulating factor;_x000D_Multipotential colony stimulating factor;_x000D_Multipotential colony-stimulating factor;_x000D_P cell stimulating factor;_x000D_P-cell-stimulating factor;_x000D_Thy1 inducing factor
  • Gen protein identity
    P01586
  • Entrez nr
    16187
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    IL-3 PicoKine
    • Gene info
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Mouse IL-3 PicoKine ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
    • Mouse
  • Technique
    ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Alternative technique
    murine, elisas, kits