- Catalog number7592-1
- Product nameThrombin, Active, Bovine Plasma (High Activity)
- Size1 KU
- PriceAsk For Price
- SynonymsActivated Factor IIa
- Alternative namesActivated Factor IIa
- DescriptionA coagulation protein and a serine protease.
- RecombinantNo
- SourceBovine plasma
- Purity by SDS PAGE≥98%
- AssaySDS-PAGE
- Biological activity>1500 units/mg powder.
- Results>1500 units/mg powder.
- Unit DefinitionActivity is expressed in NIH/US units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 ml of diluted plasma (1:1 with saline) as a Substrate and 0.1 ml of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37°C. There are approximately 5 Thrombin cleavage units for every one US Unit.
- Molecular Weight37 kDa
- Storage Temp-20°C
- ShippingGel Pack
- Shelf Life12 months
- AppearanceLyophilized
- Physical form descriptionSterile filtered and lyophilized with Mannitol and Sodium Chloride.
- Reconstitution InstructionsReconstitute in sterile water (100 U/ml) with 0.9% NaCl. It forms a clear solution.
- Background InformationThrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
- HandlingCentrifuge the vial prior to opening.
- UsageFor Research Use Only! Not to be used in humans
- Datasheet
- Gene targetThrombin Active Plasma Activity
- Short nameThrombin, Active, Plasma ( Activity)