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Testosterone (mouse/rat) ELISA Kit

  • Catalog number
    K7418-100
  • Product name
    Testosterone (mouse/rat) ELISA Kit
  • Size
    100 assays
  • Description
    Sensitive, Colorimetric Assay
  • Summary
    • Detection method- Absorbance (450 nm) • Species reactivity- Mouse/rat • Application- Quantitative protein detection, establishing normal range etc.
  • Detection Method
    Absorbance (450 nm)
  • Species Reactivity
    Mouse/rat
  • Applications
    This ELISA kit is used for quantitative protein detection, establishing normal range.
  • Sample Type
    Serum or plasma
  • Features Benefits
    • Easy, convenient and time-saving method to assay for mouse/rat Testosterone
  • Storage Conditions
    2-8°C
  • Shipping Conditions
    gel pack
  • Shelf life
    24 months
  • Background
    Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, or chidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. BioVision’s Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25 μl of Testosterone standards, controls, samples, 100 μl Testosterone-HRP conjugate reagent and 50 μl rabbit anti-testosterone reagent at room temperature for 60 min. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 min., resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Testosterone /
    • Gene info
  • Gene symbol
    CLU
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Testosterone (mouse/ ) ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
    • Mouse
  • Technique
    ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Alternative technique
    murine, elisas, kits