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PicoProbe™ ADP Assay Kit (Fluorometric)

  • Catalog number
    K211-100
  • Product name
    PicoProbe™ ADP Assay Kit (Fluorometric)
  • Size
    100 assays
  • Description
    Fast, sensitive, HTP compatible, fluorometric ADP assay with detection limit as low as 10 pmol or 0.05 µM of ADP.
  • Summary
    Ultrasensitive, Simple, HTP adaptable fluoromotericassay kit for ADP measurement. Can detect as low as 10 pmol or 0.05 µM of ADP Detection method- Fluorometric analysis (Ex/Em = 535/587 nm) Applications- Detection of NOS activity Sample Type: Tissue or cell extracts
  • Detection Method
    Fluorometric analysis (Ex/Em = 535/587 nm)
  • Species Reactivity
    Mammalian
  • Applications
    • Measurement of ADP in various tissues/cells • Analysis of metabolism and cell signaling in various cell types
  • Sample Type
    • Animal tissues - Liver, Muscle, Heart etc. • Cell culture - Adherent or suspension cells
  • Features Benefits
    Ultrasensitive, Simple, HTP adaptable fluoromotericassay kit for ADP measurement. Can detect as low as 10 pmol or 0.05 µM of ADP Detection method- Fluorometric analysis (Ex/Em = 535/587 nm) Applications- Detection of NOS activity Sample Type: Tissue or cell extracts
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Adenosine diphosphate (ADP) is an organic molecule that plays a critical role in cellular metabolism and energy transfer reactions. ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. These dephosphorylations and rephosphorylations occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes also occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is generally not available in most laboratories. BioVision’s PicoProbe™ ADP Assay Kit provides a sensitive fluorescence method to detect ADP. The kit is suitable for measuring ADP levels in various samples, including the ones that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP, in the presence of ADP Enzyme Mix is converted to an intermediate, which reacts with the fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. BioVision’s PicoProbe™ ADP Assay kit provides an ultrasensitive, fast and high-throughput ready assay for detection of as low as 10 pmol or 0.05 µM of ADP in samples.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    ™ ADP Kit Fluorometric
    • Gene info
    Gene info
  • Gene symbol
    WDTC1, ADPGK
  • Host
    Assay
  • Short name
    ™ ADP Assay Kit (Fluorometric)
  • technique filter
    • Assay
  • Technique
    Assay, assays
  • Alternative technique
    arrays, kits