Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)

• Catalog number
  K443-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple, sensitive, enzymatic assay to measurePhosphoenolpyruvate carboxylase activity in biological samples. Only kit in the market including activie positive control
• Summary
  • Detection method - Colorimetric (OD 570 nm) • Species reactivity - Mammalians • Applications - Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples, such as plants, bacteria and purified protein • Includes active PPC
• Detection Method
  Colorimetric (Absorbance 570 nm)
• Species Reactivity
  Bacterial/Plants
• Applications
  • Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples and protein preparations
• Sample Type
  • Purified protein, plant, bacterial samples
• Features Benefits
  • Simple, highly sensitive, high-throughput compatible
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phosphoenolpyruvate carboxylase (EC 4.1.1.31, PPC) is an enzyme found in plants and some types of bacteria. It catalyzes the formation of oxaloacetate from phosphoenolpyruvate by fixing one carbon in the forms of either carbon dioxide or bicarbonate. PPC participates in carbon fixation in C4 photosynthesis and Crassulacean acid metabolism (CAM) plants and acts as an anaplerotic enzyme, which replenishes TCA cycle intermediates, in bacteria and C3 plants. The participation of PPC in the carbon fixation cycle of C4 plants enable them to survive in high sunlight but dry environments. BioVision’s Phosphoenolpyruvate Carboxylase Assay Kit provides a quick and easy method for the measurement of PPC activity in various samples. In this assay, Phosphoenolpyruvate Carboxylase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converter generating a colorimetric signal (OD 570 nm). The generated absorbance signal is directly proportional to the amount of active PPC present in the samples. This kit provides a simple, fast and high throughput adaptable assay to measure the activities of PPC as low as 0.2 mU
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Phosphoenolpyruvate   Carboxylase   Activity   Kit  
• Short name
  Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Phosphoenolpyruvate Carboxylase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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