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Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)

  • Catalog number
    K443-100
  • Product name
    Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)
  • Size
    100 assays
  • Description
    Simple, sensitive, enzymatic assay to measurePhosphoenolpyruvate carboxylase activity in biological samples. Only kit in the market including activie positive control
  • Summary
    • Detection method - Colorimetric (OD 570 nm) • Species reactivity - Mammalians • Applications - Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples, such as plants, bacteria and purified protein • Includes active PPC
  • Detection Method
    Colorimetric (Absorbance 570 nm)
  • Species Reactivity
    Bacterial/Plants
  • Applications
    • Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples and protein preparations
  • Sample Type
    • Purified protein, plant, bacterial samples
  • Features Benefits
    • Simple, highly sensitive, high-throughput compatible
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Phosphoenolpyruvate carboxylase (EC 4.1.1.31, PPC) is an enzyme found in plants and some types of bacteria. It catalyzes the formation of oxaloacetate from phosphoenolpyruvate by fixing one carbon in the forms of either carbon dioxide or bicarbonate. PPC participates in carbon fixation in C4 photosynthesis and Crassulacean acid metabolism (CAM) plants and acts as an anaplerotic enzyme, which replenishes TCA cycle intermediates, in bacteria and C3 plants. The participation of PPC in the carbon fixation cycle of C4 plants enable them to survive in high sunlight but dry environments. BioVision’s Phosphoenolpyruvate Carboxylase Assay Kit provides a quick and easy method for the measurement of PPC activity in various samples. In this assay, Phosphoenolpyruvate Carboxylase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converter generating a colorimetric signal (OD 570 nm). The generated absorbance signal is directly proportional to the amount of active PPC present in the samples. This kit provides a simple, fast and high throughput adaptable assay to measure the activities of PPC as low as 0.2 mU
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Phosphoenolpyruvate Carboxylase Activity Kit
  • Host
    Assay
  • Short name
    Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)
  • technique filter
    • colorimetric
    • Assay
  • Technique
    colorimetric, Assay, assays, colorimetry
  • Alternative technique
    assays, arrays, kits