Description
For conjugation of streptavidin to proteins, peptides or sulfhydryl containing ligands. Maleimide Activated Alkaline Phosphatase is commonly used to facilitate the conjugation of alkaline phosphatase to proteins, peptides and ligands containing sulfhydryl (-SH) groups for using it as an enzyme-linked detection reagent. Maleimide Activated Alkaline Phosphatase consists of alkaline phosphatase that has been modified by hetero bi-functional cross linker Sulfo-SMCC and contains several maleimide groups per alkaline phosphatase molecule while maintaining most of the enzyme activity. After conjugation, the activated alkaline phosphatase will form a covalent interaction with the peptides or ligands containing sulfhydryl groups and can be used as a probe for detection in various assays such as ELISA, western blot etc.
