• Catalog number
    K1458-100
  • Product name
    Lentivirus and Retrovirus Purification Kit
  • Size
    100 Preps
  • Description
    Magnetic beads based kits
  • Summary
    BioVision’s Lentivirus & Retrovirus Purification Kit is used to capture, concentrate and store lentiviruses and retroviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally, viruses are purified by ultracentrifugation using CsCl, which is time consuming and is limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Lentivirus (LV)-Retrovirus (RV)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.
  • Detection Method
    Lentiviruses and Retroviruses can be captured, concentrated and stored using magnetic beads
  • Species Reactivity
    All
  • Applications
    PCR, western blot, ELISA, transfection directly with viruses bound to magnetic beads or eluted from the magnetic beads (free of beads).
  • Sample Type
    Cell and tissue culture supernatants, Serum
  • Features Benefits
    Does not need ultracentrifugation, High yield and fast concentration (2 to 1000X), Ideal for cell culture transduction or infection, Purify viruses in 30-45 min
  • Storage Conditions
    4°C
  • Shipping Conditions
    RT
  • Shelf life
    12 months
  • Background
    BioVision’s Lentivirus & Retrovirus Purification Kit is used to capture, concentrate and store lentiviruses and retroviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally, viruses are purified by ultracentrifugation using CsCl, which is time consuming and is limited to the volume of cell lysate to be processed. BioVision’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Lentivirus (LV)-Retrovirus (RV)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.
  • Data Sheet Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Lentivirus Retrovirus Kit
  • Short name
    Lentivirus Retrovirus Purification Kit
  • technique filter
    • Purification
  • Technique
    Purification, purified
  • Alternative technique
    purifications, kits
  • Virus
    retrovirus
  • virus filter
    • retrovirus