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Homocysteine Lyase Activity Assay Kit (Fluorometric)

  • Catalog number
    K2026-100
  • Product name
    Homocysteine Lyase Activity Assay Kit (Fluorometric)
  • Size
    100 assays
  • Description
    To assay HCYase activity in bacterial samples
  • Summary
    BioVision’s Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.
  • Detection Method
    Fluorescence (Ex/Em = 368/460 nm)
  • Species Reactivity
    Bacterial samples
  • Applications
    Determining the activity of wild type or recombinant Homocysteine α,γ-Lyase
  • Sample Type
    Bacterial cell lysate
  • Features Benefits
    Sensitive method to assay HCYase activity in bacterial samples, Quick, Simple
  • Storage Conditions
    -20°C
  • Shipping Conditions
    Gel Pack
  • Shelf life
    12 months
  • Background
    Homocysteine α,γ-Lyase (HCYase) is a pyridoxal 5'-phosphate dependent enzyme that catalyzes the breakdown of homocysteine to hydrogen sulfide, ammonia, and α-ketobutyrate. HCYase is present in high activity in the anaerobic protozoan parasite Trichomonas vaginalis but is absent from mammals. The Trichomonas HCYase is highly selective for homocysteine as compared to cysteine. Additionally, it has broad pH stability (pH 6-8) and is thermally stable up to 50°C. This makes rHCYase a promising candidate for therapeutic applications including the diagnosis of hyperhomocystenemia, which has been demonstrated to be a major risk factor in cardiovascular diseases. BioVision’s Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Homocysteine Lyase Activity Kit Fluorometric
  • Host
    Assay
  • Short name
    Homocysteine Lyase Activity Assay Kit (Fluorometric)
  • technique filter
    • Assay
  • Technique
    Assay, assays
  • Alternative technique
    arrays, kits