- Catalog numberK2062-200
- Product nameFirefly Luciferase Assay Kit
- Size200assays
- PriceAsk For Price
- DescriptionFor an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity
- SummaryBioVision’s Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
- Detection MethodMicroplate luminometer
- Species ReactivityMammalian
- ApplicationsDetermine the effect of inhibitors or inducers in in vitro cell-based assays.,Study promoters and its interaction with transcription factors.
- Sample TypeCell culture: adherent and suspension cells
- Features BenefitsAn accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function.,The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
- Storage Conditions-20°C
- Shipping ConditionsGel Pack
- Shelf life12 months
- BackgroundLuciferase-based assays are the most sensitive assays for studying gene regulation and function. Firefly luciferase is 100-fold more sensitive than the alternative reporters including chloramphenicol acetyltransferase, β-Glucuronidase and green fluorescent protein assays. BioVision’s Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
- Data Sheet Link
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetFirefly Luciferase Kit
- HostAssay
- Short nameFirefly Luciferase Assay Kit
- Labelluciferase
- label filter
- luciferase
- technique filter
- Assay
- TechniqueAssay, assays
- Alternative techniquearrays, kits