Firefly Luciferase Assay Kit
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Catalog number
K2062-200
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Price
Please ask
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Size
200assays
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Description
For an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity
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Summary
BioVision’s Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
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Detection Method
Microplate luminometer
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Species Reactivity
Mammalian
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Applications
Determine the effect of inhibitors or inducers in in vitro cell-based assays.,Study promoters and its interaction with transcription factors.
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Sample Type
Cell culture: adherent and suspension cells
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Features Benefits
An accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function.,The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Luciferase-based assays are the most sensitive assays for studying gene regulation and function. Firefly luciferase is 100-fold more sensitive than the alternative reporters including chloramphenicol acetyltransferase, β-Glucuronidase and green fluorescent protein assays. BioVision’s Firefly Luciferase Assay Kit is designed for an accurate, sensitive and efficient quantitation of firefly luciferase reporter enzyme activity for studying gene reporter regulation and function. In this assay, firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescent system to produce light. It is designed to detect and quantitate firefly luciferase expression in mammalian cells. The kit ensures maximum sensitivity, consistency and strong bioluminescent signal.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Firefly Luciferase Assay Kit
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Technique
Assay, assays
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Host
Assay
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Label
luciferase
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Alternative name
Firefly Luciferase test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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