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E. Coli Recombinant TRXB

  • Catalog number
    6331-100
  • Product name
    E. Coli Recombinant TRXB
  • Size
    100 ug
  • Synonyms
    E. Coli Recombinant TRXB
  • Alternative names
    Thioredoxin reductase
  • Description
    A protein involved in redox signaling
  • Recombinant
    Yes
  • Source
    E. Coli
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Biological activity
    Specific activity is 4-5 units/ml
  • Unit Definition
    Specific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm
  • Molecular Weight
    34.6 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Concentration
    1 mg/ml
  • Appearance
    Liquid
  • Physical form description
    1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1mM DTT.
  • Background Information
    Thioredoxin reductases are the only known enzymes to reduce thioredoxin. Two classes of thioredoxin reductase have been identified: one class in bacteria and some eukaryotes and one in animals. Both classes are flavoproteins which function as homodimers. TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress
  • Amino acid sequence
    MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Datasheet
    Datasheet
  • Gene target
    E TRXB
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Short name
    Recombinant TRXB
  • technique filter
    • coli recombinant
    • Recombinant
  • Technique
    coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec, escherichia