- Catalog number3057-100
- Product nameDR3 (Death Receptor 3/Apo3) Polyclonal Antibody
- Size100 µg
- PriceAsk For Price
- Antibody TargetDR3 (Death Receptor 3/Apo3)
- Target Alternative NameApo 3, LARD, TNFRSF12, TRAMP, TNFRSF25, WSL1
- Antibody TypePolyclonal
- Isotyperabbit IgG
- Species ReactivitiesHuman, Mouse, Rat
- Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acids 400 of human DR3.
- AccessionQ29905
- AppearanceColorless liquid
- FormLiquid
- Concentration0.5 mg/ml
- Formulation100 µg (0.5 mg/ml) antigen affinity-purified rabbit anti-DR3/Apo3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
- PurificationAffinity purified rabbit IgG
- ApplicationWestern blot
- Application And UsagesWestern blot: 1-4 µg
- DescriptionApoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily through interaction with their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1, Apo3, TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1, overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD, TRAF2, and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis through TRADD, FADD, and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL), thymus and spleen.
- Storage Condition-20°C
- Shipping Conditiongel pack
- Shelf Life12 months
- UsageFor Research Use Only! Not to be used in humans.
- HandlingThe antibody solution should be gently mixed before use.
- Western Blot VerifiedVerified
- Immunocytochemistry VerifiedNot Verified
- Immunofluorescence VerifiedNot Verified
- Immunoprecipitation VerifiedNot Verified
- FACS VerifiedNot Verified
- ELISA VerifiedNot Verified
- ChIP VerifiedNot Verified
- Dot Blot VerifiedNot Verified
- Flow Cytometry VerifiedNot Verified
- Data Sheet Link
- Gene targetDR3 Death Receptor 3/Apo3
- Identity:HGNC:11910
- Gene:TNFRSF25
- Long gene name:TNF receptor superfamily member 25
- Synonyms gene name:tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein), tumor necrosis factor receptor superfamily, member 25
- Synonyms:DR3, TRAMPWSL-1LARDWSL-LRDDR3TR3APO-3
- Discovery year:1998-12-04
- Entrez gene record:8718
- Pubmed identification:9052839, 8934525
- RefSeq identity:NM_148965
- Classification:Tumor necrosis factor receptor superfamily
- VEGA ID:OTTHUMG00000000831
- Gene symbolTNFRSF25
- isotype filter
- rabbit IgG
- Short nameDR3 (Death Receptor 3/Apo3) Polyclonal Antibody
- technique filter
- Polyclonal
- Antibody
- TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, antibodies